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L shotgun genomic sequences, yielded any trace of PYHIN genes in monotremes or nonmammals.Phylogenetic analysesMice were used as a source of tissues, under approval from the University of Queensland animal ethics committee (Approval number IMB/874/08). Splenocytes from C57BL/6 mice were harvested by homogenization of mouse spleens and red blood cells lysed by standard Gey's solution procedure. CD1

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